The victim had been described as a 72-year-old man, who was also in the terminal phase of pancreatic cancer.
He had been admitted to hospital on Aug. 10, with fever and tests had so far confirmed that he had listeria bacteria in his blood.
The first victim of the outbreak was a 90-year-old woman, who died on Tuesday.
However, the number of people now affected by the outbreak, which was centred on the city of Seville in southern Spain, had increased to 200, of whom 99 had been admitted to hospital and four were in intensive care.
Meanwhile, 31 of those admitted to hospital were pregnant women, one of which was highly at risk from the disease.
An infected meat product called “Mecha”, produced by a Seville-based company had been confirmed as the source of the outbreak.
The Spanish Ministry for Health, Social Services and Equality had earlier issued an international health alert over the outbreak.
The Spanish government alerted everybody as soon as the authorities in Andalusia notified of the outbreak.
“We have acted and would continue to work in line with the existing protocols,’’ the ministry said.
Meanwhile, all the meat with that trademark produced since May 2, had been immobilised, not just in Andalusia, but all over Spain.
Listeria is a bacterial infection, which is usually found in unpasteurised milk and derivative products, as well as soft cheeses and chilled ready-to-eat foods, such as sandwiches and cured meats.